Recombinant Human PDGFRB Protein

• Cat.No.: PDGFRB-050H
• Product Overview: Recombinant human Platelet-derived growth factor BB ( rhPDGF-BB ) Ser 82–Ile191 (Accession # AAH29822) was expressed in human 293 cells (HEK293).

Specification

• Description: This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008]
• Source: HEK293 cells
• Species: Human
• Molecular Mass: rhPDGF-BB is a homodimeric,glycosylated, polypeptide chain containing 2×109 amino acids, and has a calculated MW of 25 kDa. In DTT-reduced SDS-PAGE, rhPDGF-BB protein migrates as 12.8 kDa poly peptide due to glycosylation.
• Endotoxin: Less than 0.01 ng endotoxin/ 1 µg rhPDGF-BB.
• Purity: >95% as determined by SDS-PAGE. All lots are greater than 95% pure.
• Notes: Research Use Only. Not for use in diagnostic or therapeutic procedures.
• Storage: Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhSCF should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Bulk protein in a 0.22 µm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.

Gene Information

• Gene Name: PDGFRB platelet-derived growth factor receptor, beta polypeptide [ Homo sapiens ]
• Official Symbol: PDGFRB
• Synonyms: PDGFRB; platelet-derived growth factor receptor, beta polypeptide; PDGFR; platelet-derived growth factor receptor beta; CD140b; JTK12; PDGFR1; PDGFR-beta; PDGF-R-beta; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta-type platelet-derived growth factor receptor; CD140B; PDGFR-1
• Gene ID: 5159
• mRNA Refseq: NM_002609
• Protein Refseq: NP_002600
• MIM: 173410
• UniProt ID: P09619