Species : |
Human |
Source : |
HEK293 |
Tag : |
Non |
Protein Length : |
82-191 a.a. |
Description : |
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008] |
Molecular Mass : |
rhPDGF-BB is a homodimeric,glycosylated, polypeptide chain containing 2×109 amino acids, and has a calculated MW of 25 kDa. In DTT-reduced SDS-PAGE, rhPDGF-BB protein migrates as 12.8 kDa poly peptide due to glycosylation. |
Endotoxin : |
Less than 0.01 ng endotoxin/ 1 µg rhPDGF-BB. |
Purity : |
>95% as determined by SDS-PAGE. All lots are greater than 95% pure. |
Notes : |
Research Use Only. Not for use in diagnostic or therapeutic procedures. |
Storage : |
Store at -20 centigrade in lyophilized state after receipt. For long term storage, upon reconstitution rhSCF should be aliquot and store at -20 centigrade or -8 centigrade centigrade. Avoid repeated freeze-thaw cycles. |
Storage Buffer : |
Bulk protein in a 0.22 µm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization. |