Recombinant Human PDHA2 Protein, His-tagged

• Cat.No.: PDHA2-2574H
• Product Overview: Recombinant Human PDHA2 Protein (Ser28-Ser388) with a N-His tag was expressed in E. coli.

Specification

• Description: Enables pyruvate dehydrogenase (acetyl-transferring) activity. Involved in pyruvate metabolic process. Located in mitochondrion and nucleolus.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 43.6 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Ser28-Ser388
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PDHA2 pyruvate dehydrogenase (lipoamide) alpha 2 [ Homo sapiens (human) ]
• Official Symbol: PDHA2
• Synonyms: PDHA2; pyruvate dehydrogenase (lipoamide) alpha 2; PDHAL; pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; PDHE1-A type II; pyruvate dehydrogenase, E1-alpha polypeptide, testis specific; MGC149517; MGC149518
• Gene ID: 5161
• mRNA Refseq: NM_005390
• Protein Refseq: NP_005381
• MIM: 179061
• UniProt ID: P29803