Recombinant Full Length Human PDHA2 Protein, C-Flag-tagged

• Cat.No.: PDHA2-1724HFL
• Product Overview: Recombinant Full Length Human PDHA2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables pyruvate dehydrogenase (acetyl-transferring) activity. Involved in pyruvate metabolic process. Located in mitochondrion and nucleolus.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 42.8 kDa
• AA Sequence: MLAAFISRVLRRVAQKSARRVLVAS RNSSNDATFEIKKCDLYLLEEGPPV TTVLTRAEGLKYYRMMLTVR RMELKADQLYKQKFIRGFCHLCDGQ EACCVGLEAGINPSDHVITSYRAHG VCYTRGLSVRSILAELTGRR GGCAKGKGGSMHMYTKNFYGGNGIV GAQGPLGAGIALACKYKGNDEICLT LYGDGAANQGQIAEAFNMAA LWKLPCVFICENNLYGMGTSTERAA ASPDYYKRGNFIPGLKVDGMDVLCV REATKFAANYCRSGKGPILM ELQTYRYHGHSMSDPGVSYRTREEI QEVRSKRDPIIILQDRMVNSKLATV EELKEIGAEVRKEIDDAAQF
  ATTDPEPHLEELGHHIYSSDSSFEV RGANPWIKFKSVSTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis

Gene Information

• Gene Name: PDHA2 pyruvate dehydrogenase E1 subunit alpha 2 [ Homo sapiens (human) ]
• Official Symbol: PDHA2
• Synonyms: PDHAL; SPGF70
• Gene ID: 5161
• mRNA Refseq: NM_005390.5
• Protein Refseq: NP_005381.1
• MIM: 179061
• UniProt ID: P29803