Recombinant Human PDHB Protein, His-tagged

• Cat.No.: PDHB-135H
• Product Overview: Recombinant Human PDHB, transcript variant 2, fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene.
• Form: 25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.
• Molecular Mass: 38 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: PDHB pyruvate dehydrogenase (lipoamide) beta [ Homo sapiens ]
• Official Symbol: PDHB
• Synonyms: PDHB; pyruvate dehydrogenase (lipoamide) beta; pyruvate dehydrogenase E1 component subunit beta, mitochondrial; pyruvate dehydrogenase, E1 beta polypeptide; PDHBD; PHE1B; PDHE1-B; DKFZp564K0164
• Gene ID: 5162
• mRNA Refseq: NM_001173468
• Protein Refseq: NP_001166939
• MIM: 179060
• UniProt ID: P11177