Recombinant Human PDZ and LIM domain 1, His-tagged

• Cat.No.: PDLIM1-430H
• Product Overview: PDLIM1, 1-329aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-329 a.a.
• Description: PDLIM1 is a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. Recombinant human PDLIM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT, 2mM EDTA
• Molecular Mass: 38.7 kDa (354aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMTTQQ IDLQGPGPWG FRLVGGKDFE QPLAISRVTP GSKAALANLC IGDVITAIDG ENTSNMTHLE AQNRIKGCTD NLTLTVARSE HKVWSPLVTE EGKRHPYKMN LASEPQEVLH IGSAHNRSAM PFTASPASST TARVITNQYN NPAGLYSSEN ISNFNNALES KTAASGVEAN SRPLDHAQPP SSLVIDKESE VYKMLQEKQE LNEPPKQSTS FLVLQEILES EEKGDPNKPS GFRSVKAPVT KVAASIGNAQ KLPMCDKCGT GIVGVFVKLR DRHRHPECYV CTDCGTNLKQ KGHFFVEDQI YCEKHARERV TPPEGYEVVT VFPK
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PDLIM1 PDZ and LIM domain 1 [ Homo sapiens ]
• Official Symbol: PDLIM1
• Synonyms: PDLIM1; PDZ and LIM domain 1; CLIM1, PDZ and LIM domain 1 (elfin); PDZ and LIM domain protein 1; carboxyl terminal LIM domain protein 1; CLP 36; CLP36; elfin; hCLIM1; enigma; LIM domain protein CLP-36; C-terminal LIM domain protein 1; carboxy terminal LIM domain protein 1; CLIM1; CLP-36
• Gene ID: 9124
• mRNA Refseq: NM_020992
• Protein Refseq: NP_066272
• MIM: 605900
• UniProt ID: O00151
• Chromosome Location: 10q23.1
• Function: metal ion binding; transcription coactivator activity; zinc ion binding