Recombinant Human PFKFB4, GST-tagged

• Cat.No.: PFKFB4-216H
• Product Overview: Recombinant human PFKFB4 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PFKFB4 (also known as 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4) regulates the steady-state concentration of fructose-2, 6-bisphosphate, a potent activator of a key regulatory enzyme of glycolysis pathway called phosphofructokinase. Isozymes of PFKFB differ in the regions surrounding the catalytic core, which are important for the differential response to allosteric effectors and hormonal signals in different tissues. PFKFB4 is highly expressed in brain, heart, liver, muscle, placenta, adipose tissue, ovary, fallopian tubes, and testis.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~82 kDa
• Purity: >95% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: PFKFB4 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 [ Homo sapiens ]
• Official Symbol: PFKFB4
• Synonyms: PFKFB4; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; PFK/FBPase 4; 6PF-2-K/Fru-2,6-P2ase 4; 6PF-2-K/Fru-2,6-P2ase testis-type isozyme; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4; bifunctional enzyme with kinase and biphosphatase activities
• Gene ID: 5210
• mRNA Refseq: NM_004567
• Protein Refseq: NP_004558
• MIM: 605320
• UniProt ID: Q16877
• Chromosome Location: 3p22-p21
• Pathway: Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycolysis, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of carbohydrates, organism-specific biosystem
• Function: 6-phosphofructo-2-kinase activity; 6-phosphofructo-2-kinase activity; ATP binding; fructose-2,6-bisphosphate 2-phosphatase activity; hydrolase activity; identical protein binding; kinase activity; nucleotide binding; transferase activity