Recombinant Human PFKL Protein, His-tagged

• Cat.No.: PFKL-2581H
• Product Overview: Recombinant Human PFKL Protein (Ala2-His390) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 45.7 kDa
  Accurate Molecular Mass: 43 kDa
• Protein length: Ala2-His390
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PFKL phosphofructokinase, liver [ Homo sapiens (human) ]
• Official Symbol: PFKL
• Synonyms: PFKL; phosphofructokinase, liver; 6-phosphofructokinase, liver type; phosphohexokinase; phosphofructokinase 1; liver-type 1-phosphofructokinase; phosphofructo-1-kinase isozyme B; PFK-B; FLJ30173; FLJ40909; DKFZp686G1648; DKFZp686L2097
• Gene ID: 5211
• mRNA Refseq: NM_002626
• Protein Refseq: NP_002617
• MIM: 171860
• UniProt ID: P17858