Recombinant Human PHGDH protein, His-tagged

• Cat.No.: PHGDH-852H
• Product Overview: Recombinant Human PHGDH protein(O43175) (Met 1-Phe 533) was expressed in E.coli, fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-533 aa
• Form: Lyophilized from sterile PBS, 100mM Arg, 0.1% Tween20, 20% glycerol, pH 8.0.
• Molecular Mass: The recombinant human PHGDH comprises 543 amino acids and has a calculated molecular mass of 58KDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PHGDH phosphoglycerate dehydrogenase [ Homo sapiens ]
• Official Symbol: PHGDH
• Synonyms: PHGDH; phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; PDG; PGDH; SERA; 3-phosphoglycerate dehydrogenase; PGD; PGAD; 3PGDH; 3-PGDH; MGC3017
• Gene ID: 26227
• mRNA Refseq: NM_006623
• Protein Refseq: NP_006614
• MIM: 606879
• UniProt ID: O43175