Recombinant Human PHLPP1 protein, GST-tagged

• Cat.No.: PHLPP1-107H
• Product Overview: Recombinant Human PHLPP1 protein(NP_919431)(1068-1204 aa), fused to GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
• Protein length: 1068-1204 aa
• AA Sequence: QQHLLQVPAEASDEGIVISANEDEP GLPRKADFSAVGTIGRRRANGSVAP QERSHNVIEVATDAPLRKPGGYFAA PAQPDPDDQFIIPPELEEEVKEIMK HHQEQQQQQQPPPPPQLQPQLPRHY QLDQLPDYYDTP
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
  Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.

Gene Information

• Gene Name: PHLPP1 PH domain and leucine rich repeat protein phosphatase 1 [ Homo sapiens ]
• Official Symbol: PHLPP1
• Synonyms: PHLPP1; PH domain and leucine rich repeat protein phosphatase 1; PH domain and leucine rich repeat protein phosphatase , PHLPP, pleckstrin homology domain containing, family E (with leucine rich repeats) member 1 , PLEKHE1; PH domain leucine-rich repeat-containing protein phosphatase 1; KIAA0606; SCOP; SCN circadian oscillatory protein; PH domain-containing family E member 1; suprachiasmatic nucleus circadian oscillatory protein; pleckstrin homology domain containing, family E (with leucine rich repeats) member 1; PHLPP; PLEKHE1; MGC161555
• Gene ID: 23239
• mRNA Refseq: NM_194449
• Protein Refseq: NP_919431
• MIM: 609396
• UniProt ID: O60346