Recombinant Human Phosphatase, Orphan 2, His-tagged

• Cat.No.: PHOSPHO2-1574H
• Product Overview: Recombinant human PHOSPHO2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Pyridoxal phosphate phosphatase PHOSPHO2, orphan 2, also known as PHOSPHO2, belongs to the haloaciddehalogenase(HAD) superfamily. Phosphatase has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralization. PHOSPHO2 is a putative phosphatase sharing 42% sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are very similar, but surprisingly, recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine relatively poorly.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing1mM DTT, 10% glycerol, 0.1M NaCl.
• Molecular Weight: 30.3 kDa (265aa), confirmed by MALDI-TOF.
• Purity: >85% by SDS-PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMKILLV FDFDNTIIDD NSDTWIVQCA PNKKLPIELR DSYRKGFWTE FMGRVFKYLG DKGVREHEMKRAVTSLPFTP GMVELFNFIR KNKDKFDCII ISDSNSVFID WVLEAASFHD IFDKVFTNPA AFNSNGHLTV ENYHTHSCNR CPKNLCKKVV LIEFVDKQLQ QGVNYTQIVY IGDGGNDVCP VTFLKNDDVA MPRKGYTLQK TLSRMSQNLE PMEYSVVVWS SGVDIISHLQ FLIKD
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Metabolic pathways; Vitamin B6 metabolism

Gene Information

• Gene Name: PHOSPHO2 phosphatase, orphan 2 [ Homo sapiens ]
• Official Symbol: PHOSPHO2
• Synonyms: PHOSPHO2; phosphatase, orphan 2; MGC22679; MGC111048; pyridoxal phosphate phosphatase PHOSPHO2; OTTHUMP00000204769; OTTHUMP00000204770; OTTHUMP00000204771; OTTHUMP00000204772; EC 3.1.3.74; MGC111048
• Gene ID: 493911
• mRNA Refseq: NM_001008489
• Protein Refseq: NP_001008489
• UniProt ID: Q8TCD6
• Chromosome Location: 2q31.1
• Function: hydrolase activity; metal ion binding; pyridoxal phosphatase activity