Recombinant Human PIK3C2A Protein (Phe172-Asn403), His tagged

• Cat.No.: PIK3C2A-3165H
• Product Overview: Recombinant human PIK3C2A (Phe172-Asn403) protein fused with His tag was expressed in E. coli.

Specification

• Description: Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3, 4-bisphosphate (PtdIns (3, 4) P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 27.12 kDa
• Protein length: Phe172-Asn403
• Purity: >90 % as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS pH 7.5 or lyophilized from PBS pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: PIK3C2A phosphoinositide-3-kinase, class 2, alpha polypeptide [ Homo sapiens (human) ]
• Official Symbol: PIK3C2A
• Synonyms: PIK3C2A; phosphoinositide-3-kinase, class 2, alpha polypeptide; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha; PI3K C2alpha; PI3K-C2alpha; PI3K-C2-alpha; ptdIns-3-kinase C2 subunit alpha; phosphoinositide 3-kinase-C2-alpha; C2-containing phosphatidylinositol kinase; CPK; PI3-K-C2A; MGC142218; PI3-K-C2 (ALPHA); DKFZp686L193
• Gene ID: 5286
• mRNA Refseq: NM_002645
• Protein Refseq: NP_002636
• MIM: 603601
• UniProt ID: O00443

Reviews

10/22/2020

The PIK3C2A protein is known for its exceptional quality, making it a highly suitable choice for meeting diverse experimental needs.

08/23/2020

Its versatility and reliability make it an indispensable asset for scientists seeking to explore the complexities of various biological systems.

01/27/2019

Its stability and functional attributes ensure reliable imaging and facilitate the elucidation of complex protein structures.

Q&As

Can targeting PIK3C2A have potential applications in immunotherapy?

PIK3C2A inhibitors may enhance the efficacy of immunotherapy by modulating immune cell function and overcoming resistance to immune checkpoint inhibitors in cancer.

What strategies are being explored to target PIK3C2A for the treatment of insulin resistance and diabetes?

Research is focusing on developing small molecule inhibitors and gene therapy approaches to modulate PIK3C2A activity as a potential therapeutic strategy for diabetes.

What role does PIK3C2A play in the development of neurological disorders?

PIK3C2A has been implicated in the pathogenesis of neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease, suggesting its potential as a target for disease-modifying therapies.

What are the potential therapeutic applications of targeting PIK3C2A in cancer treatment?

Targeting PIK3C2A may lead to the development of novel anticancer therapies, including small molecule inhibitors and monoclonal antibodies.

How does PIK3C2A protein contribute to cell signaling pathways?

PIK3C2A is involved in the PI3K/Akt/mTOR signaling pathway, which regulates cell growth, proliferation, and survival, making it an attractive target for therapeutic intervention.