Recombinant Human PIK3CA Protein, His-tagged

Cat.No. : PIK3CA-2591H
Product Overview : Recombinant Human PIK3CA Protein (Ile330-Pro487) with a N-His tag was expressed in E. coli.
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Description : Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
Source : E. coli
Species : Human
Tag : N-His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 21.6 kDa
Accurate Molecular Mass: 22 kDa
Protein length : Ile330-Pro487
Endotoxin : <1.0 EU per 1µg (determined by the LAL method).
Purity : > 95%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide [ Homo sapiens (human) ]
Official Symbol : PIK3CA
Synonyms : PIK3CA; phosphoinositide-3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3K; PI3K-alpha; PI3-kinase p110 subunit alpha; ptdIns-3-kinase subunit p110-alpha; serine/threonine protein kinase PIK3CA; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; p110-alpha; MGC142161; MGC142163;
Gene ID : 5290
mRNA Refseq : NM_006218
Protein Refseq : NP_006209
MIM : 171834
UniProt ID : P42336

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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