Active Recombinant Human PIKFYVE protein, GST-tagged

• Cat.No.: PIKFYVE-173H
• Product Overview: Recombinant Human PIKFYVE fused with an N-terminal GST tag was expressed in Insect cells.

Specification

• Description: PIP5K3 (also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) encodes an enzyme that phosphorylates the D-5 position in PtdIns and phospha-tidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns (3,5)biphosphate. PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol but also have protein kinase activity. PIP5K3 regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. The inhibition of PIP5K3 renders the late endosome /lysosome compartment refractory to fusion with autop-hagosomes and multivesicular bodies.
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 110 nmol/min/mg
• Molecular Mass: 110 kDa
• Purity: >85%
• Applications: Kinase Assay
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PIKFYVE phosphoinositide kinase, FYVE-type zinc finger containing [ Homo sapiens ]
• Official Symbol: PIKFYVE
• Synonyms: CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29; PIPkin-III; epididymis luminal protein 37; phosphatidylinositol 3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III; phosphoinositide kinase, FYVE finger containing; type III PIP kinase; zinc finger, FYVE domain containing 29
• Gene ID: 200576
• mRNA Refseq: NM_015040
• Protein Refseq: NP_055855
• MIM: 609414
• UniProt ID: Q9Y2I7
• Chromosome Location: 2q34
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; PI Metabolism, organism-specific biosystem; Synthesis of PIPs at the Golgi membrane, organism-specific biosystem
• Function: 1-phosphatidylinositol-3-phosphate 5-kinase activity; NOT protein binding involved in protein folding; NOT unfolded protein binding