Recombinant Human Pirin (iron-binding nuclear protein), His-tagged

• Cat.No.: PIR-5547H
• Product Overview: Recombinant humanPIR protein,fused to His-tag at N-terminus, was expressed in E. coli and purified byusing conventional chromatography.

Specification

• Species: Human
• Tag: His
• Description: PIR, also known aspirin, is a memberof the cupin superfamily. PIR is an Fe(II)-containing nuclear proteinexpressed in all tissues of the body and concentrated within dot-likesubnuclear structures. Interactions with nuclear factor I/CCAAT boxtranscription factor as well as B cell lymphoma 3-encoded oncoprotein suggestthe encoded protein may act as a transcriptional cofactor and be involved inthe regulation of DNA transcription and replication.
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
• Molecular Weight: 34.3 kDa (310aa)confirmed by MALDI-TOF
• Purity: > 95%by SDS-PAGE
• Concentration: 1 mg/ml (determinedby Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGRPGGFPDHPHRG FETVSYLLEG GSMAHEDFCG HTGKMNPGDL QWMTAGRGIL HAEMPCSEEP AHGLQLWVNLRSSEKMVEPQ YQELKSEEIP KPSKDGVTVA VISGEALGIK SKVYTRTPTL YLDFKLDPGA KHSQPIPKGWTSFIYTISGD VYIGPDDAQQ KIEPHHTAVL GEGDSVQVEN KDPKRSHFVL IAGEPLREPV IQHGPFVMNTNEEISQAILD FRNAKNGFER AKTWKSKIGN
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoidrepeated freezing and thawing cycles.

Gene Information

• Gene Name: PIR pirin (iron-binding nuclearprotein) [ Homo sapiens ]
• Official Symbol: PIR
• Synonyms: PIR;pirin (iron-binding nuclear protein); pirin; probablequercetinase; probable quercetin 2,3-dioxygenase PIR
• Gene ID: 8544
• mRNA Refseq: NM_001018109
• Protein Refseq: NP_001018119
• MIM: 603329
• UniProt ID: O00625
• Chromosome Location: Xp22.2
• Function: metal ion binding;oxidoreductase activity; protein binding; quercetin 2,3-dioxygenase activity;transcription cofactor activity