Recombinant Human PKMYT1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PKMYT1-5959H
• Product Overview: PKMYT1 MS Standard C13 and N15-labeled recombinant protein (NP_004194) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 54.3 kDa
• AA Sequence: MLERPPALAMPMPTEGTPPPLSGTP IPVPAYFRHAEPGFSLKRPRGLSRS LPPPPPAKGSIPISRLFPPRTPGWH QLQPRRVSFRGEASETLQSPGYDPS RPESFFQQSFQRLSRLGHGSYGEVF KVRSKEDGRLYAVKRSMSPFRGPKD RARKLAEVGSHEKVGQHPCCVRLEQ AWEEGGILYLQTELCGPSLQQHCEA WGASLPEAQVWGYLRDTLLALAHLH SQGLVHLDVKPANIFLGPRGRCKLG DFGLLVELGTAGAGEVQEGDPRYMA PELLQGSYGTAADVFSLGLTILEVA CNMELPHGGEGWQQLRQGYLPPEFT AGLSSELRSVLVMMLEPDPKLRATA EALLALPVLRQPRAWGVLWCMAAEA LSRGWALWQALLALLCWLWHGLAHP ASWLQPLGPPATPPGSPPCSLLLDS SLSSNWDDDSLGPSLSPEAVLARTV GSTSTPRSRCTPRDALDLSDINSEP PRGSFPSFEPRNLLSLFEDTLDPTT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PKMYT1 protein kinase, membrane associated tyrosine/threonine 1 [ Homo sapiens (human) ]
• Official Symbol: PKMYT1
• Synonyms: PKMYT1; protein kinase, membrane associated tyrosine/threonine 1; membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; MYT1; myt1 kinase; FLJ20093; DKFZp547K1610
• Gene ID: 9088
• mRNA Refseq: NM_004203
• Protein Refseq: NP_004194
• MIM: 602474
• UniProt ID: Q99640