Recombinant Human PLA2G1B, His-tagged

• Cat.No.: PLA2G1B-898H
• Product Overview: Recombinant Human PLA2G1B protein (Met 1 - Ser 148), fused with a his tag at the C-terminus, was expressed inHuman Cells.

Specification

• Species: Human
• Source: Human Cells
• Tag: His
• Protein Length: 1-148 a.a.
• Description: Phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein which belongs to the phospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes.
• Molecular Mass: The recombinant human PLA2G1B comprises 144 amino acids after removal of the signal peptide, and has a predicted molecular mass of 16.2 kDa. rh PLA2G1B migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Asp 16.
• Purity: > 92 % as determined by SDS-PAGE.
• Formulation: Lyophilized from sterile 10mM Tris, 5mM CaCl, pH 8.0.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃.
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: PLA2G1B phospholipase A2, group IB (pancreas) [ Homo sapiens ]
• Synonyms: PLA2G1B; PLA2G1B phospholipase A2, group IB (pancreas); PLA2; PLA2A; PPLA2; MGC119834; MGC119835; phospholipase A2 group IB; phosphatidylcholine 2-acylhydrolase
• Gene ID: 5319
• mRNA Refseq: NM_000928
• Protein Refseq: NP_000919
• MIM: 172410
• UniProt ID: P04054
• Chromosome Location: 12q23-q24.1
• Pathway: Arachidonic acid metabolism; Ether lipid metabolism; Fc epsilon RI signaling pathway; Linoleic acid metabolism; Long-term depression; MAPK signaling pathway; Metabolic pathways; VEGF signaling pathway; Vascular smooth muscle contraction; alpha-Linolenic acid metabolism; Glycerophospholipid metabolism; GnRH signaling pathway
• Function: bile acid binding; calcium ion binding; calcium-dependent phospholipase A2 activity; cell surface binding; hydrolase activity; receptor binding