Active Recombinant Human PLAU protein(activated by trypsin), His-tagged

• Cat.No.: PLAU-389H
• Product Overview: Recombinant Human PLAU protein(NP_002649.1) (Met 1-Leu 431) with a C-terminal polyhistidine tag was expressed in HEK293. The purified protein was activated by trypsin in vitro.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-431 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant human PLAU comprises 422 amino acids with a predicted molecular mass of 46 kDa. As a result of glycosylation and cleavage, rhPLAU migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length PLAU with the molecular mass of 18, 32 and 50 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PLAU plasminogen activator, urokinase [ Homo sapiens ]
• Official Symbol: PLAU
• Synonyms: PLAU; plasminogen activator, urokinase; urokinase-type plasminogen activator; UPA; URK; U-plasminogen activator; plasminogen activator, urinary; ATF; QPD; u-PA; BDPLT5
• Gene ID: 5328
• mRNA Refseq: NM_001145031
• Protein Refseq: NP_001138503
• MIM: 191840
• UniProt ID: P00749