Active Recombinant Human PLAUR protein, His-tagged

• Cat.No.: PLAUR-392H
• Product Overview: Active Recombinant Human PLAUR protein(Q03405)(13~305 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 13-305 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Urokinase-type plasminogen activator receptor (uPAR) is a glycosylphosphatidylinositol (GPI)-anchored protein. Besides regulating proteolysis, uPAR could also activate many intracellular signaling pathways that promote cell motility, invasion, proliferation and survival through cooperating with transmembrane receptors. uPAR is overexpressed across a variety of tumors and is associated with cancer invasion and metastasis. ITGb1 has been identified as an interactor of uPAR, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human uPAR and recombinant human ITGb1. Briefly, uPAR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ITGb1-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-uPAR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human uPAR and recombinant human ITGb1 was shown, the EC50 for this effect is 0.165 ug/mL.
• Molecular Mass: 35.9kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 80% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PLAUR plasminogen activator, urokinase receptor [ Homo sapiens ]
• Official Symbol: PLAUR
• Synonyms: PLAUR; plasminogen activator, urokinase receptor; urokinase plasminogen activator surface receptor; CD87; UPAR; URKR; urokinase type plasminogen activator (uPA) receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; urokinase-type plasminogen activator (uPA) receptor; U-PAR
• Gene ID: 5329
• mRNA Refseq: NM_001005376
• Protein Refseq: NP_001005376
• MIM: 173391
• UniProt ID: Q03405