Recombinant Human PLCD4 Protein (1-762 aa), His-SUMO-tagged

• Cat.No.: PLCD4-1123H
• Product Overview: Recombinant Human PLCD4 Protein (1-762 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Full Length.

Specification

• Description: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 103.6 kDa
• Protein length: 1-762 aa
• AA Sequence: MASLLQDQLTTDQDLLLMQEGMPMR KVRSKSWKKLRYFRLQNDGMTVWHA RQARGSAKPSFSISDVETIRNGHDS ELLRSLAEELPLEQGFTIVFHGRRS NLDLMANSVEEAQIWMRGLQLLVDL VTSMDHQERLDQWLSDWFQRGDKNQ DGKMSFQEVQRLLHLMNVEMDQEYA FSLFQAADTSQSGTLEGEEFVQFYK ALTKRAEVQELFESFSADGQKLTLL EFLDFLQEEQKERDCTSELALELID RYEPSDSGKLRHVLSMDGFLSYLCS KDGDIFNPACLPIYQDMTQPLNHYF ICSSHNTYLVGDQLCGQSSVEGYIR ALKRGCRCVEVDVWDGPSGEPVVYH GHTLTSRILFKDVVATVAQYAFQTS DYPVILSLETHCSWEQQQTMARHLT EILGEQLLSTTLDGVLPTQLPSPEE LRRKILVKGKKLTLEEDLEYEEEEA EPELEESELALESQFETEPEPQEQN LQNKDKKKKSKPILCPALSSLVIYL KSVSFRSFTHSKEHYHFYEISSFSE TKAKRLIKEAGNEFVQHNTWQLSRV YPSGLRTDSSNYNPQELWNAGCQMV AMNMQTAGLEMDICDGHFRQNGGCG YVLKPDFLRDIQSSFHPEKPISPFK AQTLLIQVISGQQLPKVDKTKEGSI VDPLVKVQIFGVRLDTARQETNYVE NNGFNPYWGQTLCFRVLVPELAMLR FVVMDYDWKSRNDFIGQYTLPWTCM QQGYRHIHLLSKDGISLRPASIFVY ICIQEGLEGDES
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: PLCD4 phospholipase C, delta 4 [ Homo sapiens ]
• Official Symbol: PLCD4
• Synonyms: PLCD4; PLC delta4; MGC12837
• Gene ID: 84812
• mRNA Refseq: NM_032726
• Protein Refseq: NP_116115
• MIM: 605939
• UniProt ID: Q9BRC7