Recombinant Human PLCE1 Protein, His&GST-tagged

• Cat.No.: PLCE1-1948H
• Product Overview: Recombinant Human PLCE1 Protein (Met1-Gln250) with N-His&GST tag was expressed in E. coli.

Specification

• Description: This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: E. coli
• Species: Human
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 58.1 kDa
  Accurate Molecular Mass: 58 kDa
• Protein length: Met1-Gln250
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: PLCE1 phospholipase C epsilon 1 [ Homo sapiens (human) ]
• Official Symbol: PLCE1
• Synonyms: PLCE1; phospholipase C epsilon 1; PLCE; PPLC; NPHS3; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; PLC-epsilon-1; pancreas-enriched phospholipase C; phosphoinositide phospholipase C; phosphoinositide phospholipase C-epsilon-1; phosphoinositide-specific phospholipase C epsilon-1; EC 3.1.4.11
• Gene ID: 51196
• mRNA Refseq: NM_001165979
• Protein Refseq: NP_001159451
• MIM: 608414
• UniProt ID: Q9P212