Recombinant Human PLCG1 Protein, His-tagged

• Cat.No.: PLCG1-334H
• Product Overview: Recombinant Human PLCG1(Ser1091~Leu1290) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 26.5kDa
• Identity: Reconstitute in PBS or others
• Protein length: Ser1091~Leu1290
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: PLCG1 phospholipase C, gamma 1 [ Homo sapiens ]
• Official Symbol: PLCG1
• Synonyms: PLCG1; phospholipase C, gamma 1; phospholipase C, gamma 1 (formerly subtype 148) , PLC1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; NCKAP3; PLC II; PLC148; PLCgamma1; PLC-148; PLC-gamma-1; phospholipase C-II; phospholipase C-148; phosphoinositidase C; phospholipase C-gamma-1; inositoltrisphosphohydrolase; phosphoinositide phospholipase C; phosphatidylinositol phospholipase C; triphosphoinositide phosphodiesterase; phosphoinositide phospholipase C-gamma-1; monophosphatidylinositol phosphodiesterase; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; phospholipase C, gamma 1 (formerly subtype 148); 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; PLC1; PLC-II
• Gene ID: 5335
• mRNA Refseq: NM_002660
• Protein Refseq: NP_002651
• MIM: 172420
• UniProt ID: P19174