Recombinant Human PLCZ1 protein, His/sumo-tagged

• Cat.No.: PLCZ1-137H
• Product Overview: Recombinant Human PLCZ1(1-415aa) fused with His/sumotag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the phosphoinositide-specific phospholipase C family. Members in this family, classified into six isotypes that are tissue- and organ-specific, hydrolyze phosphatidylinositol 4,5-bisphosphate just before the phosphate group to yield diacylglycerol and inositol 1,4,5-trisphosphate. This protein localizes to the acrosome in spermatozoa and elicits Ca(2+) oscillations and egg activation during fertilization that leads to early embryonic development. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His/sumo
• Form: Tris-based buffer,50% glycerol
• Protein length: 1-415aa
• AA Sequence: MEMRWFLSKIQDDFRGGKINLEKTQ RLLEKLDIRCSYIHVKQIFKTSDYP VVLSLENHCSTAQQEVMADNLQATF GESLLSDMLDDFPDTLPSPEALKFK ILVKNKKIGTLKETHERKGSDKRGD NQDKETGVKKLPGVMLFKKKKTRKL KIALALSDLVIYTKAEKFKSFQHSR LYQQFNENNSIGETQARKLSKLRVH EFIFHTRKFITRIYPKATRADSSNF NPQEFWNIGCQMVALNFQTPGLPMD LQNGKFLDNGGSGYILKPHFLRESK SYFNPSNIKEGMPITLTIRLISGIQ LPLTHSSSNKGDSLVIIEVFGVPND QMKQQTRVIKKNAFSPRWNETFTFI IHVPELALIRFVVEGQGLIAGNEFL GQYTLPLLCMNKGYRRIPLFSRMGE SLEPASLFVYVWYVR
• Purity: >90% ( SDS-PAGE )
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: Store at -20 centigrade, for extended storage, conserve at -20 centigrade or -80 centigrade.

Gene Information

• Gene Name: PLCZ1 phospholipase C, zeta 1 [ Homo sapiens ]
• Official Symbol: PLCZ1
• Synonyms: PLCZ1; phospholipase C, zeta 1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1; NYD SP27; PLCzeta; PLC-zeta-1; phospholipase C-zeta-1; PI-phospholipase C zeta 1; testis-development protein NYD-SP27; testis-development related NYD-SP27; phosphoinositide phospholipase C-zeta-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1; NYD-SP27; MGC149685
• Gene ID: 89869
• mRNA Refseq: NM_033123
• Protein Refseq: NP_149114
• MIM: 608075
• UniProt ID: Q86YW0
• Chromosome Location: 12p13.31
• Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, organism-specific biosystem; D-myo-inositol-5-phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Inositol phosphate metabolism, PI=>
• Function: calcium ion binding; hydrolase activity; phosphatidylinositol phospholipase C activity; signal transducer activity