| Description : |
The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
95.8 kDa |
| AA Sequence : |
MERAESSSTEPAKAIKPIDRKSVHQ ICSGQVVLSLSTAVKELVENSLDAG ATNIDLKLKDYGVDLIEVSDNGCGV EEENFEGLTLKHHTSKIQEFADLTQ VETFGFRGEALSSLCALSDVTISTC HASAKVGTRLMFDHNGKIIQKTPYP RPRGTTVSVQQLFSTLPVRHKEFQR NIKKEYAKMVQVLHAYCIISAGIRV SCTNQLGQGKRQPVVCTGGSPSIKE NIGSVFGQKQLQSLIPFVQLPPSDS VCEEYGLSCSDALHNLFYISGFISQ CTHGVGRSSTDRQFFFINRRPCDPA KVCRLVNEVYHMYNRHQYPFVVLNI SVDSECVDINVTPDKRQILLQEEKL LLAVLKTSLIGMFDSDVNKLNVSQQ PLLDVEGNLIKMHAADLEKPMVEKQ DQSPSLRTGEEKKDVSISRLREAFS LRHTTENKPHSPKTPEPRRSPLGQK RGMLSSSTSGAISDKGVLRPQKEAV SSSHGPSDPTDRAEVEKDSGHGSTS VDSEGFSIPDTGSHCSSEYAASSPG DRGSQEHVDSQEKAPETDDSFSDVD CHSNQEDTGCKFRVLPQPTNLATPN TKRFKKEEILSSSDICQKLVNTQDM SASQVDVAVKINKKVVPLDFSISSL AKRIKQLHHEAQQSEGEQNYRKFRA KICPGENQAAEDELRKEISKTMFAE MEIIGQFNLGFIITKLNEDIFIVDQ HATDEKYNFEMLQQHTVLQGQRLIA PQTLNLTAVNEAVLIENLEIFRKNG FDFVIDENAPVTERAKLISLPTSKN WTFGPQDVDELIFMLSDSPGVMCRP SRVKQMFASRACRKSVMIGTALNTS EMKKLITHMGEMDHPWNCPHGRPTM RHIANLGVISQNTRTRPLEQKLISE EDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
