Recombinant Human POLA1 protein, His & T7-tagged

• Cat.No.: POLA1-8020H
• Product Overview: Recombinant Human POLA1 aa. (Gly1240~Ser1462 (Accession # P09884)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.5kDa
• Protein length: Gly1240~Ser1462 (Accession # P09884)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.6.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: POLA1 DNA polymerase alpha 1, catalytic subunit [ Homo sapiens (human) ]
• Official Symbol: POLA1
• Synonyms: NSX; POLA; p180; DNA polymerase alpha catalytic subunit p180; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); DNA polymerase alpha catalytic subunit
• Gene ID: 5422
• mRNA Refseq: NM_001330360.1
• Protein Refseq: NP_001317289.1
• UniProt ID: P09884