Recombinant Human POLI Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human POLI Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : POLI-1020H
Product Overview : POLI MS Standard C13 and N15-labeled recombinant protein (NP_009126) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 80.3 kDa
AA Sequence : MELADVGAAA SSQGVHDQVL PTPNASSRVI VHVDLDCFYA QVEMISNPEL KDKPLGVQQK YLVVTCNYEA RKLGVKKLMN VRDAKEKCPQ LVLVNGEDLT RYREMSYKVT ELLEEFSPVV ERLGFDENFV DLTEMVEKRL QQLQSDELSA VTVSGHVYNN QSINLLDVLH IRLLVGSQIA AEMREAMYNQ LGLTGCAGVA SNKLLAKLVS GVFKPNQQTV LLPESCQHLI HSLNHIKEIP GIGYKTAKCL EALGINSVRD LQTFSPKILE KELGISVAQR IQKLSFGEDN SPVILSGPPQ SFSEEDSFKK CSSEVEAKNK IEELLASLLN RVCQDGRKPH TVRLIIRRYS SEKHYGRESR QCPIPSHVIQ KLGTGNYDVM TPMVDILMKL FRNMVNVKMP FHLTLLSVCF CNLKALNTAK KGLIDYYLMP SLSTTSRSGK HSFKMKDTHM EDFPKDKETN RDFLPSGRIE STRTRESPLD TTNFSKEKDI NEFPLCSLPE GVDQEVFKQL PVDIQEEILS GKSREKFQGK GSVSCPLHAS RGVLSFFSKK QMQDIPINPR DHLSSSKQVS SVSPCEPGTS GFNSSSSSYM SSQKDYSYYL DNRLKDERIS QGPKEPQGFH FTNSNPAVSA FHSFPNLQSE QLFSRNHTTD SHKQTVATDS HEGLTENREP DSVDEKITFP SDIDPQVFYE LPEAVQKELL AEWKRAGSDF HIGHKTRTRP LEQKLISEED LAANDILDYK DDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : POLI DNA polymerase iota [ Homo sapiens (human) ]
Official Symbol : POLI
Synonyms : POLI; polymerase (DNA directed) iota; RAD3OB, RAD30B; DNA polymerase iota; eta2; RAD30 homolog B; polymerase (DNA-directed), iota; RAD30B; RAD3OB;
Gene ID : 11201
mRNA Refseq : NM_007195
Protein Refseq : NP_009126
MIM : 605252
UniProt ID : Q9UNA4

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