Recombinant Human Polo-Like Kinase 1, GST-tagged

• Cat.No.: PLK2-1086H
• Product Overview: Full length recombinant human PLK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PLK2 or Polo-like kinase 2 is a serum-inducible kinase that is a member of the Polo family of serine/threonine protein kinases which play an essential role in normal cell division. PLK2 phosphorylates the P4.1-associated protein (CPAP), a human homologue of SAS-4, in procentriole formation during the centrosome cycle. PLK2 may also play a role as a tumor suppressor since it interacts with TSC1 protein which in turn inhibits mTOR signaling, tumor growth and chemosensitivity under hypoxic conditions. Human lung tumor cells deficient in PLK2 grow larger than control tumors, and PLK2 interacts with endogenous TSC1 protein in these tumors.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 106 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM Tris-HCL, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 0 25% glycerol.
• Specific Activity: 13 nmol/min/mg
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: PLK2 and PLK4 events; Polo-like kinase signaling events in the cell cycle
• Full Length: Full L.

Gene Information

• Gene Name: PLK2 polo-like kinase 2 [ Homo sapiens ]
• Official Symbol: PLK2
• Synonyms: PLK2; polo-like kinase 2; SNK; serine/threonine-protein kinase PLK2; PLK-2; OTTHUMP00000122549; serum-inducible kinase; serine/threonine-protein kinase SNK
• Gene ID: 10769
• mRNA Refseq: NM_006622
• Protein Refseq: NP_006613
• MIM: 607023
• UniProt ID: Q9NYY3
• Chromosome Location: 5q12.1-q13.2
• Function: ATP binding; nucleotide binding; protein serine/threonine kinase activity; signal transducer activity