| Description : |
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
60.6 kDa |
| AA Sequence : |
MTQAEIKLCSLLLQEHFGEIVEKIG VHLIRTGSQPLRVIAHDTGTSLDQV KKALCVLVQHNLVSYQVHKRGVVEY EAQCSRVLRMLRYPRYIYTTKTLYS DTGELIVEELLLNGKLTMSAVVKKV ADRLTETMEDGKTMDYAEVSNTFVR LADTHFVQRCPSVPTTENSDPGPPP PAPTLVINEKDMYLVPKLSLIGKGK RRRSSDEDAAGEPKAKRPKYTTDNK EPIPDDGIYWQANLDRFHQHFRDQA IVSAVANRMDQTSSEIVRTMLRMSE ITTSSSAPFTQPLSSNEIFRSLPVG YNISKQVLDQYLTLLADDPLEFVGK SGDSGGGMYVINLHKALASLATATL ESVVQERFGSRCARIFRLVLQKKHI EQKQVEDFAMIPAKEAKDMLYKMLS ENFMSLQEIPKTPDHAPSRTFYLYT VNILSAARMLLHRCYKSIANLIERR QFETKENKRLLEKSQRVEAIIASMQ ATGAEEAQLQEIEEMITAPERQQLE TLKRNVNKLDASEIQVDETIFLLES YIECTMKRQTRTRPLEQKLISEEDL AANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
