Recombinant Human POLR3F protein, His-tagged

• Cat.No.: POLR3F-1851H
• Product Overview: Recombinant Human POLR3F protein(1-316 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-316 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPIIPPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: POLR3F polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa [ Homo sapiens ]
• Official Symbol: POLR3F
• Synonyms: POLR3F; polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa; polymerase (RNA) III (DNA directed) polypeptide F (39 kDa); DNA-directed RNA polymerase III subunit RPC6; RNA polymerase III C39 subunit; RPC6; RPC39; RNA polymerase III subunit C6; RNA polymerase III 39 kDa subunit; DNA-directed RNA polymerase III subunit F; DNA-directed RNA polymerase III 39 kDa polypeptide; DNA-directed RNA polymerases III 39 kDa polypeptide; MGC13517
• Gene ID: 10621
• mRNA Refseq: NM_006466
• Protein Refseq: NP_006457
• UniProt ID: Q9H1D9