Recombinant Human Polyglutamine Binding Protein 1, His-tagged

• Cat.No.: PQBP1-6773H
• Product Overview: Recombinant humanPQBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Polyglutaminebinding protein 1, also known as PQBP1, is a transcription repressor thatassociates with polyglutamine tract-containing transcription regulators andcausative genes for neurodegenerative disorders. PQBP-1 localizes to thenucleus and is present in neurons throughout the brain, with abundant levelsin hippocampus, cerebellar cortex and olfactory bulb. PQBP-1 contains aWWP/WW domain that binds proline-rich motifs and a C2 domain that caninfluence Ca2+-dependent phospholipid signaling
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT
• Molecular Weight: 33 kDa (289aa),confirmed by MALDI-TOF
• Purity: > 95% by SDS -PAGE
• Concentration: 0.5 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMPLPVA LQTRLAKRGI LKHLEPEPEE EIIAEDYDDD PVDYEATRLE GLPPSWYKVFDPSCGLPYYWNADTDLVSWL SPHDPNSVVT KSAKKLRSSN ADAEEKLDRS HDKSDRGHDK SDRSHEKLDRGHDKSDRGHD KSDRDRERGY DKVDRERERD RERDRDRGYD KADREEGKER RHHRREELAP YPKSKKAVSRKDEELDPMDP SSYSDAPRGT WSTGLPKRNE AKTGADTTAA GPLFQQRPYP SPGAVLRANA EASRTKQQD
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PQBP1 polyglutamine bindingprotein 1 [Homo sapiens]
• Official symbol: PQBP1
• Synonyms: polyglutaminebinding protein 1; SHS; NPW38; mental retardation, X-linked 55; Polyglutaminetract-binding protein 1; Sutherland-Haan X-linked mental retardationsyndrome; 38 kDa nuclear protein containing a WW domain; OTTHUMP00000025809;OTTHUMP00000025812; MRX55; nuclear protein containing WW domain 38 kD; MRXS3;polyglutamine-binding protein 1; MRXS8; RENS1; PQBP-1; OTTHUMP00000025808
• Gene ID: 10084
• mRNA Refseq: NM_005710
• Protein Refseq: NP_005701
• MIM: 300463
• Uniprot ID: O60828
• Chromosome Location: Xp11.23
• Pathway: Spliceosome, organism-specific biosystem; Spliceosome, conservedbiosystem
• Function: DNA binding; transcription coactivator activity