Recombinant Human Polymerase (RNA) III (DNA Sirected) Polypeptide H (22.9kD), His-tagged

• Cat.No.: POLR3H-7187H
• Product Overview: Recombinant human POLR3H protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 25.3kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-204aa
• Description: POLR3H, also as known as A1665 and RPC8, belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. POLR3H acts as nuclear and cytosolic DNA sensor involved in innate immune response.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT.
• Molecular Mass: 25.3kDa (227aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMFVLVEM VDTVRIPPWQ FERKLNDSIA EELNKKLANK VVYNVGLCIC LFDITKLEDA YVFPGDGASH TKVHFRCVVF HPFLDEILIG KIKGCSPEGV HVSLGFFDDI LIPPESLQQP AKFDEAEQVW VWEYETEEGA HDLYMDTGEE IRFRVVDESF VDTSPTGPSS ADATTSSEEL PKKEAPYTLV GSISEPGLGL LSWWTSN
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: POLR3H polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) [ Homo sapiens ]
• Official Symbol: POLR3H
• Synonyms: POLR3H; polymerase (RNA) III (DNA directed) polypeptide H (22.9kD); DNA-directed RNA polymerase III subunit RPC8; KIAA1665; RPC8; RNA polymerase III subunit C8; RNA polymerase III subunit RPC8; DNA-directed RNA polymerase III subunit H; RNA nucleotidyltransferase (DNA-directed); RNA polymerase III subunit 22.9 kDa subunit; DNA-directed RNA polymerase III subunit 22.9 kDa polypeptide; RPC22.9; MGC29654; MGC111097
• Gene ID: 171568
• mRNA Refseq: NM_001018050
• Protein Refseq: NP_001018060
• UniProt ID: Q9Y535
• Chromosome Location: 22q13
• Pathway: Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Epstein-Barr virus infection, organism-specific biosystem; Epstein-Barr virus infection, conserved biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Gene Expression, organism-specific biosystem; Metabolic pathways, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity