Recombinant Human PON1 Protein, His-tagged


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Recombinant Human PON1 Protein, His-tagged

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Cat.No. : PON1-01H
Product Overview : Recombinant human PON1 (16-355) protein with a His-tag was expressed in HEK293.
Description : This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7.
Source : HEK293
Species : Human
Tag : His
Form : Liquid
Bio-activity : Specific activity is > 2,500 pmol/min/µg, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37 centigrade.
Molecular Mass : 39 kDa
Protein length : 16-355
Endotoxin : < 1 EU per 1 µg of protein (determined by LAL method)
Purity : > 90% by SDS-PAGE
Applications : SDS-PAGE, Enzyme Activity
Storage : Stored at +2 centigrade to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles
Concentration : 0.25 mg/ml
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
SDS-PAGE Analysis of PON1 :

3 µg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Gene Name : PON1 paraoxonase 1 [ Homo sapiens (human) ]
Official Symbol : PON1
Synonyms : PON1; paraoxonase 1; ESA; PON; MVCD5; serum paraoxonase/arylesterase 1; A-esterase 1; K-45; PON 1; aromatic esterase 1; arylesterase 1; arylesterase B-type; esterase A; paraoxonase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1
Gene ID : 5444
mRNA Refseq : NM_000446
Protein Refseq : NP_000437
MIM : 168820
UniProt ID : P27169
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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