Recombinant Human PON1 Protein, His/Fc-tagged


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Recombinant Human PON1 Protein, His/Fc-tagged

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Cat.No. : PON1-32H
Product Overview : Recombinant human PON1 protein (Leu16-Leu355) with an N-terminal 6-His tag and a C-terminal Fc tag was expressed in Human embryonic kidney cell.
Description : This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7.
Source : HEK293
Species : Human
Tag : His/Fc
Bio-activity : Measured by its ability to hydrolyze phenyl acetate. The specific activity is >5000 pmol/min/μg.
Molecular Mass : 66 kDa
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening.
Storage Buffer : Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl, and Glycerol.
Figure :

2 μg/lane of Recombinant Human PON1 His-tag Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing a band at ~74 kDa under reducing conditions.

Gene Name : PON1 paraoxonase 1 [ Homo sapiens (human) ]
Official Symbol : PON1
Synonyms : PON1; paraoxonase 1; ESA; PON; MVCD5; serum paraoxonase/arylesterase 1; A-esterase 1; K-45; PON 1; aromatic esterase 1; arylesterase 1; arylesterase B-type; esterase A; paraoxonase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; EC; EC; EC
Gene ID : 5444
mRNA Refseq : NM_000446
Protein Refseq : NP_000437
MIM : 168820
UniProt ID : P27169

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