Recombinant Human PPCDC, His-tagged

• Cat.No.: PPCDC-2841H
• Product Overview: Recombinant human PPCDC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =24.6 kDa (224aa), confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PPCDC belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily that plays a role in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B). The biosynthesis of CoA from pantothenate requires several steps: the phosphorylation of pantothenate, the conversion of 4'-hosphopantothenate to 4"-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC plays a direct role in this pathway by converting 4"-phosphopantothenate into 4"-phosphopantetheine.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS
• Purity: > 95 % by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PPCDC phosphopantothenoylcysteine decarboxylase [ Homo sapiens ]
• Synonyms: PPCDC; phosphopantothenoylcysteine decarboxylase; MDS018; FLJ14585; coaC; PPC-DC; EC 4.1.1.36
• Gene ID: 60490
• mRNA Refseq: NM_021823
• Protein Refseq: NP_068595
• MIM: 609854
• UniProt ID: Q96CD2
• Chromosome Location: 15q24.2
• Pathway: Metabolic pathways; Pantothenate and CoA biosynthesis
• Function: lyase activity; phosphopantothenoylcysteine decarboxylase activity