Recombinant Human PPP1CB Protein, His-tagged

• Cat.No.: PPP1CB-2614H
• Product Overview: Recombinant Human PPP1CB Protein (Phe45-Arg327) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36 kDa
  Accurate Molecular Mass: 36 kDa
• Protein length: Phe45-Arg327
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PPP1CB protein phosphatase 1, catalytic subunit, beta isozyme [ Homo sapiens (human) ]
• Official Symbol: PPP1CB
• Synonyms: PPP1CB; protein phosphatase 1, catalytic subunit, beta isozyme; protein phosphatase 1, catalytic subunit, beta isoform; serine/threonine-protein phosphatase PP1-beta catalytic subunit; PP 1B; PP1beta; protein phosphatase 1-beta; protein phosphatase 1-delta; protein phosphatase 1, catalytic subunit, delta isoform; serine/threonine protein phosphatase PP1-beta catalytic subunit; PP-1B; PPP1CD; MGC3672
• Gene ID: 5500
• mRNA Refseq: NM_002709
• Protein Refseq: NP_002700
• MIM: 600590
• UniProt ID: P62140