| Description : |
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
78.4 kDa |
| AA Sequence : |
MAGLGPGVGDSEGGPRPLFCRKGAL RQKVVHEVKSHKFTARFFKQPTFCS HCTDFIWGIGKQGLQCQVCSFVVHR RCHEFVTFECPGAGKGPQTDDPRNK HKFRLHSYSSPTFCDHCGSLLYGLV HQGMKCSCCEMNVHRRCVRSVPSLC GVDHTERRGRLQLEIRAPTADEIHV TVGEARNLIPMDPNGLSDPYVKLKL IPDPRNLTKQKTRTVKATLNPVWNE TFVFNLKPGDVERRLSVEVWDWDRT SRNDFMGAMSFGVSELLKAPVDGWY KLLNQEEGEYYNVPVADADNCSLLQ KFEACNYPLELYERVRMGPSSSPIP SPSPSPTDPKRCFFGASPGRLHISD FSFLMVLGKGSFGKVMLAERRGSDE LYAIKILKKDVIVQDDDVDCTLVEK RVLALGGRGPGGRPHFLTQLHSTFQ TPDRLYFVMEYVTGGDLMYHIQQLG KFKEPHAAFYAAEIAIGLFFLHNQG IIYRDLKLDNVMLDAEGHIKITDFG MCKENVFPGTTTRTFCGTPDYIAPE IIAYQPYGKSVDWWSFGVLLYEMLA GQPPFDGEDEEELFQAIMEQTVTYP KSLSREAVAICKGFLTKHPGKRLGS GPDGEPTIRAHGFFRWIDWERLERL EIPPPFRPRPCGRSGENFDKFFTRA APALTPPDRLVLASIDQADFQGFTY VNPDFVHPDARSPTSPVPVPVMTRT RPLEQKLISEEDLAANDILDYKDDD DKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
