Description : |
This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
67.3 kDa |
AA Sequence : |
MSHTVAGGGSGDHSHQVRVKAYYRG DIMITHFEPSISFEGLCNEVRDMCS FDNEQLFTMKWIDEEGDPCTVSSQL ELEEAFRLYELNKDSELLIHVFPCV PERPGMPCPGEDKSIYRRGARRWRK LYCANGHTFQAKRFNRRAHCAICTD RIWGLGRQGYKCINCKLLVHKKCHK LVTIECGRHSLPQEPVMPMDQSSMH SDHAQTVIPYNPSSHESLDQVGEEK EAMNTRESGKASSSLGLQDFDLLRV IGRGSYAKVLLVRLKKTDRIYAMKV VKKELVNDDEDIDWVQTEKHVFEQA SNHPFLVGLHSCFQTESRLFFVIEY VNGGDLMFHMQRQRKLPEEHARFYS AEISLALNYLHERGIIYRDLKLDNV LLDSEGHIKLTDYGMCKEGLRPGDT TSTFCGTPNYIAPEILRGEDYGFSV DWWALGVLMFEMMAGRSPFDIVGSS DNPDQNTEDYLFQVILEKQIRIPRS MSVKAASVLKSFLNKDPKERLGCLP QTGFADIQGHPFFRNVDWDMMEQKQ VVPPFKPNISGEFGLDNFDSQFTNE RVQLTPDDDDIVRKIDQSEFEGFEY INPLLMSAEECVTRTRPLEQKLISE EDLAANDILDYKDDDDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |