Recombinant Human PRKCI Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PRKCI-3713H
• Product Overview: PRKCI MS Standard C13 and N15-labeled recombinant protein (NP_002731) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 67.3 kDa
• AA Sequence: MSHTVAGGGSGDHSHQVRVKAYYRG DIMITHFEPSISFEGLCNEVRDMCS FDNEQLFTMKWIDEEGDPCTVSSQL ELEEAFRLYELNKDSELLIHVFPCV PERPGMPCPGEDKSIYRRGARRWRK LYCANGHTFQAKRFNRRAHCAICTD RIWGLGRQGYKCINCKLLVHKKCHK LVTIECGRHSLPQEPVMPMDQSSMH SDHAQTVIPYNPSSHESLDQVGEEK EAMNTRESGKASSSLGLQDFDLLRV IGRGSYAKVLLVRLKKTDRIYAMKV VKKELVNDDEDIDWVQTEKHVFEQA SNHPFLVGLHSCFQTESRLFFVIEY VNGGDLMFHMQRQRKLPEEHARFYS AEISLALNYLHERGIIYRDLKLDNV LLDSEGHIKLTDYGMCKEGLRPGDT TSTFCGTPNYIAPEILRGEDYGFSV DWWALGVLMFEMMAGRSPFDIVGSS DNPDQNTEDYLFQVILEKQIRIPRS MSVKAASVLKSFLNKDPKERLGCLP QTGFADIQGHPFFRNVDWDMMEQKQ VVPPFKPNISGEFGLDNFDSQFTNE RVQLTPDDDDIVRKIDQSEFEGFEY INPLLMSAEECVTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PRKCI protein kinase C iota [ Homo sapiens (human) ]
• Official Symbol: PRKCI
• Synonyms: PRKCI; protein kinase C, iota; DXS1179E; protein kinase C iota type; PKCI; PRKC-lambda/iota; aPKC-lambda/iota; atypical protein kinase C-lambda/iota; nPKC-iota; MGC26534
• Gene ID: 5584
• mRNA Refseq: NM_002740
• Protein Refseq: NP_002731
• MIM: 600539
• UniProt ID: P41743