Recombinant Human PRKG1 protein, His-tagged

• Cat.No.: PRKG1-5633H
• Product Overview: Recombinant Human PRKG1 protein(1-178 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-178 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQAQKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPG
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: PRKG1 protein kinase, cGMP-dependent, type I [ Homo sapiens ]
• Official Symbol: PRKG1
• Synonyms: PRKG1; protein kinase, cGMP-dependent, type I; PRKG1B, PRKGR1B; cGMP-dependent protein kinase 1; PGK; PKG; protein kinase, cGMP-dependent, regulatory, type I, beta; 1; cGK; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha; FLJ36117; MGC71944; DKFZp686K042
• Gene ID: 5592
• mRNA Refseq: NM_001098512
• Protein Refseq: NP_001091982
• MIM: 176894
• UniProt ID: Q13976