Active Recombinant Human PRLR protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: PRLR-135H
• Product Overview: Biotinylated Recombinant Human PRLR protein(P16471-1)(25-234 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 25-234 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human PRLR, His Avi Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.32 kDa. Due to glycosylation, the protein migrates to 32-40 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: PRLR prolactin receptor [ Homo sapiens ]
• Official Symbol: PRLR
• Synonyms: PRLR; prolactin receptor; PRL-R; hPRL receptor; secreted prolactin binding protein; hPRLrI
• Gene ID: 5618
• mRNA Refseq: NM_000949
• Protein Refseq: NP_000940
• MIM: 176761
• UniProt ID: P16471