PRMT3-007H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Human PRMT3 protein (2-531) with a N-terminal GST-tag was expressed in E. coli.
Description :
This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants.
Source :
E. coli
Species :
Human
Tag :
GST
Bio-activity :
Activity was determined using a radiometric assay with [3H]-S-adenosylmethionine incubated with core histones or the glycine arginine rich (GAR) region from fibrillarin at 30 centigrade for 30 minutes or overnight at room temperature in buffer containing 50 mM Tris, pH 8.0, 150 mM sodium chloride, 3 mM MgCl2 , and 5% glycerol. Methylated products were separated by SDS-PAGE and visualized by exposure on autoradiographic film.
Molecular Mass :
86.6 kDa
Protein length :
2-531
Purity :
≥90% estimated by SDS-PAGE
Stability :
≥ 6 months
Storage :
-80 centigrade
Storage Buffer :
50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride and 20% glycerol
SDS-PAGE Analysis of PRMT3 :
Lane 1: PRMT3 (3 μM) with GAR peptide Lane 2: PRMT3 (3 μM) with GAR peptide Lane 3: PRMT3 (3 μM) with Core Histones Lane 4: PRMT3 (3 μM) with Core Histones
Analysis of PRMT3 :
Lane 1: MW Markers Lane 2: PRMT3 (2.5 μg) Lane 3: PRMT3 (5 μg)
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.