Active Recombinant Human PRMT3 Protein, GST-tagged

• Cat.No.: PRMT3-007H
• Product Overview: Recombinant Human PRMT3 protein (2-531) with a N-terminal GST-tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 2-531
• Description: This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants.
• Bio-activity: Activity was determined using a radiometric assay with [3H]-S-adenosylmethionine incubated with core histones or the glycine arginine rich (GAR) region from fibrillarin at 30 centigrade for 30 minutes or overnight at room temperature in buffer containing 50 mM Tris, pH 8.0, 150 mM sodium chloride, 3 mM MgCl2 , and 5% glycerol. Methylated products were separated by SDS-PAGE and visualized by exposure on autoradiographic film.
• Molecular Mass: 86.6 kDa
• Purity: ≥90% estimated by SDS-PAGE
• Stability: ≥ 6 months
• Storage: -80 centigrade
• Storage Buffer: 50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride and 20% glycerol

Gene Information

• Gene Name: PRMT3 protein arginine methyltransferase 3 [ Homo sapiens (human) ]
• Official Symbol: PRMT3
• Synonyms: PRMT3; protein arginine methyltransferase 3; HRMT1L3; protein arginine N-methyltransferase 3; HMT1 hnRNP methyltransferase-like 3; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
• Gene ID: 10196
• mRNA Refseq: NM_005788
• Protein Refseq: NP_005779
• MIM: 603190
• UniProt ID: O60678