Active Recombinant Human PROCR protein, His-GST-tagged

• Cat.No.: PROCR-568H
• Product Overview: Active Recombinant Human PROCR protein(Q9UNN8)(18~210 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: 18-210 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Endothelial protein C receptor (EPCR) also known as CD201, is a transmembrane glycoprotein expressed on vascular endothelial cells and functions as a negative regulator of thrombosis. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Mature human EPCR consists of a 193 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail. Within the ECD, human EPCR shares 63% and 66% aa sequence identity with mouse and rat EPCR, respectively. EPCR inhibits thrombosis through its interactions with Protein C, activated Protein C (APC), and Coagulation Factors VII, and VIIa. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EPCR and recombinant rat Coagulation Factor VII (F7). Briefly, EPCR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to F7-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-EPCR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human EPCR and recombinant rat F7 was shown, the EC50 for this effect is 0.06 ug/mL.
• Molecular Mass: 52.0kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 80% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PROCR protein C receptor, endothelial [ Homo sapiens ]
• Official Symbol: PROCR
• Synonyms: PROCR; protein C receptor, endothelial; endothelial protein C receptor; CCD41; CD201; EPCR; APC receptor; centrocyclin; CD201 antigen; activated protein C receptor; endothelial cell protein C receptor; cell cycle, centrosome-associated protein; CCCA; bA42O4.2; MGC23024
• Gene ID: 10544
• mRNA Refseq: NM_006404
• Protein Refseq: NP_006395
• MIM: 600646
• UniProt ID: Q9UNN8