Recombinant Human Proliferating Cell Nuclear Antigen

• Cat.No.: PCNA-536H
• Product Overview: Recombinant human Proliferating Cell Nuclear Antigen, was expressed inE.coliand purified by conventional chromatography techniques. MW = 28 kDa (261 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighbouring tumours.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 2 mM EDTA 20% glycerol.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLR SEG FDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVF EAP NQE K VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLN FFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publications:
  Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture (2017)

Gene Information

• Gene Name: PCNA proliferating cell nuclear antigen [ Homo sapiens ]
• Synonyms: MGC8367; PCNA; Proliferating cell nuclear antigen; Cyclin; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
• Gene ID: 5111
• mRNA Refseq: NM_002592
• Protein Refseq: NP_002583
• MIM: 176740
• UniProt ID: P12004
• Chromosome Location: 20pter-p12
• Pathway: Base excision repair; Cell cycle; DNA replication; Mismatch repair; Nucleotide excision repair; Cell Cycle, Mitotic; DNA Repair; DNA Replication; Telomere Maintenance
• Function: DNA binding; DNA polymerase processivity factor activity; MutLalpha complex binding; dinucleotide insertion or deletion binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity