Recombinant Human Proteasome (prosome, macropain) Subunit, Beta Type, 6, His-tagged

• Cat.No.: PSMB6-1566H
• Product Overview: Recombinant human PSMB6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PSMB6 is a member of the proteasome B-type family, also known as the T1B family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by PSMB9. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
• Molecular Weight: 24.2 kDa (226aa), confirmed by MALDI-TOF.
• Purity: >85% by SDS-PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MTTIMAVQFD GGVVLGADSR TTTGSYIANR VTDKLTPIHD RIFCCRSGSA ADTQAVADAV TYQLGFHSIE LNEPPLVHTA ASLFKEMCYR YREDLMAGII IAGWDPQEGG QVYSVPMGGM MVRQSFAIGG SGSSYIYGYV DATYREGMTK EECLQFTANA LALAMERDGS SGGVIRLAAI AESGVERQVL LGDQIPKFAV ATLPPA
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of Cdh1 by Cdh1:APC/C; CDK-mediated phosphorylation and removal of Cdc6; CDT1 association with the CDC6:ORC:origin complex; Cell Cycle Checkpoints; Cyclin A:Cdk2-associated events at S phase entry; DNA Replication; G1/S DNA Damage Checkpoints; p53-Independent G1/S DNA damage checkpoint; Vpu mediated degradation of CD4; Ubiquitin-dependent degradation of Cyclin D1

Gene Information

• Gene Name: PSMB6 proteasome (prosome, macropain) subunit, beta type, 6 [ Homo sapiens ]
• Official Symbol: PSMB6
• Synonyms: PSMB6; proteasome (prosome, macropain) subunit, beta type, 6; Y; Multicatalyticendopeptidase complex delta chain; DELTA; Macropain delta chain; Proteasome subunit Y; Proteasome delta chain; LMPY; proteasome subunit beta 6; proteasome subunit beta type-6; proteasome subunit delta; MGC5169; proteasome catalytic subunit 1; PSY large multifunctional protease Y; EC 3.4.25.1; OTTHUMP00000115982
• Gene ID: 5694
• mRNA Refseq: NM_002798
• Protein Refseq: NP_002789
• MIM: 600307
• UniProt ID: P28072
• Chromosome Location: 17p13
• Function: endopeptidase activity; peptidase activity; threonine-type endopeptidase activity