Recombinant Human Protein Kinase, AMP-Activated, Beta 1 Non-Catalytic Subunit, His-tagged

• Cat.No.: PRKAB1-4868H
• Product Overview: Recombinant full-length human PRKAB1 (combination of A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: PRKAB1 (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes. PRKAB1 is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of PRKAB1 in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 69kDa (A2), 38kDa (B1), and 40kDa (G1).
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Specific Activity: 270 nmol/min/mg
• Purity: > 75 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: AMPK signaling; Adipocytokine signaling pathway; Direct p53 effectors; Energy Metabolism; Energy dependent regulation of mTOR by LKB1-AMPK; Hypertrophic cardiomyopathy (HCM); IRS-mediated signalling; IRS-related events; Insulin receptor signalling cascade; Insulin signaling pathway; PI3K Cascade; PKB-mediated events; Regulation of AMPK activity via LKB1; Regulation of Rheb GTPase activity by AMPK; Signaling by Insulin receptor; mTOR signalling

Gene Information

• Gene Name: PRKAB1 protein kinase, AMP-activated, beta 1 non-catalytic subunit [ Homo sapiens ]
• Official Symbol: PRKAB1
• Synonyms: PRKAB1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; AMPK; HAMPKb; MGC17785; 5"-AMP-activated protein kinase subunit beta-1; AMPKb; AMPK beta 1; AMPK beta -1 chain; OTTHUMP00000239813; OTTHUMP00000239814; AMPK subunit beta-1; AMP-activated protein kinase beta subunit; 5"-AMP-activated protein kinase beta-1 subunit; protein kinase, AMP-activated, noncatalytic, beta-1
• Gene ID: 5564
• mRNA Refseq: NM_006253
• Protein Refseq: NP_006244
• MIM: 602740
• UniProt ID: Q9Y478
• Chromosome Location: 12q24.1-q24.3
• Function: AMP-activated protein kinase activity; cAMP-dependent protein kinase activity; protein binding; protein kinase binding