Recombinant Human Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A, His-tagged

• Cat.No.: PPP1R14A-110H
• Product Overview: Recombinant His tagged PPP1R14A was expressed in E.coli and purified by using conventional chromatography techniques (167 aa, 18 kDa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 2 mM EDTA, 10% glycerol.
• Molecular Weight: 18 kDa (167 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MGSSHHHHHH SSGLVPRGSH MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PPP1R14A
• Synonyms: PPP1R14A; protein phosphatase 1, regulatory (inhibitor) subunit 14A; CPI17; CPI-17; PPP1INL; 17 kDa PKC-potentiated inhibitory protein of PP1; PPP1R14A protein phosphatase 1, regulatory (inhibitor) subunit 14A; 17-KDa protein; 17-kDa PKC-potentiated inhibitory protein of PP1; PKC-potentiated inhibitory protein of PP1.
• Gene ID: 94274
• mRNA Refseq: NM_033256.1
• Protein Refseq: NP_150281.1
• MIM: 608153
• UniProt ID: Q96A00
• Chromosome Location: 19q13.1
• Function: phosphoprotein phosphatase inhibitor activity; protein binding.