Recombinant Human Protein Phosphatase 3, Regulatory Subunit B, His-tagged

• Cat.No.: PPP3R2-973H
• Product Overview: Recombinant human PPP3R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PPP3R2 is a calcium-dependent, calmodulin stimulated serine/threonine protein phosphatase. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Recombinant human PPP3R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Formulation: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT.
• Molecular Weight: 22 kDa (193aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS – PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of animo acids: MGSSHHHHHH SSGLVPRGSHMSTMGNEASY PAEMCSHFDN DEIKRLGRRF KKLDLDKSGS LSVEEFMSLP ELRHNPLVRR VIDVFDTDGD GEVDFKEFIL GTSQFSVKGD EEQKLRFAFSIYDMDKDGYI SNGELFQVLK MMVGNNLTDW QLQQLVDKTI IILDKDGDGK ISFEEFSAVV RDLEIHKKLV LIV
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PPP3R2 protein phosphatase 3, regulatory subunit B, beta [ Homo sapiens ]
• Synonyms: PPP3R2;PPP3RL; protein phosphatase 3, regulatory subunit B, beta; Calcineurin subunit B type 2;Calcineurin B-like protein; Calcineurin BII; Protein phosphatase 2B regulatory subunit 2; Protein phosphatase 3 regulatory subunit B beta isoform; CBLP; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), beta isoform (calcineurin B, type II);protein phosphatase 3 (formerly 2B), regulatory subunit B, beta isoform
• Gene ID: 5535
• mRNA Refseq: NM_147180
• Protein Refseq: NP_671709
• UniProt ID: Q96LZ3
• Chromosome Location: 9q31
• Function: calcium ion binding