Recombinant Human Protein Tyrosine Phosphatase, Receptor Type, N, GST-tagged

• Cat.No.: PTPRN-1307H
• Product Overview: Recombinanthuman PTPRN is expressed in E. coli; it contains the catalyticdomain of PTPRN (residues 693-979), with aN-terminal GST tag, and has a molecular weight of 58.8 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 693-979 a.a.
• Description: PTPRN (proteintyrosine phosphatase-IA2; also known as ICA-512), together with PTP-IAR,belongs to a subgroup of receptor-like protein tyrosine phosphatases. PTPRNwas found to be an autoantigen that is reactive with insulin-dependentdiabetes mellitus (IDDM) patient sera, and thus may be a potential target ofautoimmunity in diabetes mellitus. It is highly expressed in neuroendocrinetissues. Human PTPRN is a receptor protein tyrosine phosphatase containing979 amino acids.
• Form: 25 mMTris-HCl (pH 8.0), 75 mM NaCl, 0.05% Tween-20, 2 mM EDTA, 1 mM DTT and 50%glycerol. Liquid at -20°C.
• Purity: Greaterthan 95% as determined by Coomassie-stained SDS-PAGE gel.
• Specific Activity: 1.4U/ug. One unit will hydrolyze 1 pmol of 6, 8-difluoro-4-methyl umbelliferylphosphate (DiFMUP) per minute at pH 6.3 and 30°C. Assay buffer: 50 mMBis-Tris (pH 6.3), 2 mM EDTA, 2 mM DTT and 100 mM DiFMUP.
• Applications: Removal ofphosphates from target substrates. Useful for the study of enzyme kinetics,regulation and for inhibitors screening.
• Storage: 3 monthsat -20°C.
• OfficialSymbol: PTPRN
• Pathways: Type I diabetesmellitus

Gene Information

• Gene Name: PTPRN protein tyrosine phosphatase,receptor type, N [ Homo sapiens ]
• Synonyms: PTPRN; proteintyrosine phosphatase, receptor type, N; IA2; IA-2; ICA512;R-PTP-N; IA-2/PTP; receptor-type tyrosine-protein phosphatase-like N; ICA 512;PTP IA-2; OTTHUMP00000164170; OTTHUMP00000205824; islet cell antigen 2; isletcell antigen 512; islet cell autoantigen 3; protein tyrosine phosphatase-likeN
• Gene ID: 5798
• mRNA Refseq: NM_001199763
• Protein Refseq: NP_001186692
• MIM: 601773
• UniProt ID: Q16849
• Chromosome Location: 2q35-q36.1
• Function: GTPase binding;hydrolase activity; phosphatase activity; protein binding; protein tyrosinephosphatase activity; receptor activity; transmembrane receptor proteintyrosine phosphatase activity