Recombinant Human PRPF18 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PRPF18-2350H
• Product Overview: PRPF18 MS Standard C13 and N15-labeled recombinant protein (NP_003666) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18.
• Molecular Mass: 39.9 kDa
• AA Sequence: MDILKSEILRKRQLVEDRNLLVENKKYFKRSELAKKEEEAYFERCGYKIQPKEEDQKPLTSSNPVLELELAEEKLPMTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLRKIEILTPEVNKGLRNDLKAALDKIDQQYLNEIVGGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDDHKDMDIITKFLKFLLGVWAKELNAREDYVKRSVQGKLNSATQKQTESYLRPLFRKLRKRNLPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKHVAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNALTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PRPF18 pre-mRNA processing factor 18 [ Homo sapiens (human) ]
• Official Symbol: PRPF18
• Synonyms: PRPF18; PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae); PRP18 pre mRNA processing factor 18 homolog (yeast); pre-mRNA-splicing factor 18; hPrp18; PRP18 homolog; PRP18 pre-mRNA processing factor 18 homolog(PRPF18); PRP18; FLJ10210
• Gene ID: 8559
• mRNA Refseq: NM_003675
• Protein Refseq: NP_003666
• MIM: 604993
• UniProt ID: Q99633