Recombinant Human PRUNE2 protein, His-tagged

• Cat.No.: PRUNE2-1998H
• Product Overview: Recombinant Human PRUNE2 protein(1-259 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-259 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: MEEFLQRAKSKLNRSKRLEKVHVVIGPKSCDLDSLISTFTYAYFLDKVSPPGVLCLPVLNIPRTEFNYFTETRFILEELNISESFHIFRDEINLHQLNDEGKLSITLVGSSVLASEDKTLESAVVKVINPVEQSDANVEFRESSSSLVLKEILQEAPELITEQLAHRLRGSILFKWMTMESEKISEKQEEILSILEEKFPNLPPREDIINVLQETQFSAQGLSIEQTMLKDLKELSDGEIKVAISTVSMNLEVRVGMLF
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: PRUNE2 prune homolog 2 (Drosophila) [ Homo sapiens ]
• Official Symbol: PRUNE2
• Synonyms: PRUNE2; prune homolog 2 (Drosophila); C9orf65, chromosome 9 open reading frame 65 , KIAA0367; protein prune homolog 2; A214N16.3; bA214N16.3; BMCC1; BNIPXL; truncated PRUNE2; BNIP2 motif-containing molecule at the C-terminal region 1; BCH motif-containing molecule at the carboxyl terminal region 1; BNIP2 motif containing molecule at the carboxyl terminal region 1; C9orf65; KIAA0367; RP11-58J3.2; FLJ50060; FLJ54876; FLJ59118; DKFZp762K117
• Gene ID: 158471
• mRNA Refseq: NM_015225
• Protein Refseq: NP_056040
• MIM: 610691
• UniProt ID: Q8WUY3