Description : |
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that functions as a 20S core alpha subunit. The encoded protein interacts with the hepatitis B virus X protein and plays a role in regulating hepatitis C virus internal ribosome entry site (IRES) activity, an activity essential for viral replication. The encoded protein also plays a role in the cellular stress response by regulating hypoxia-inducible factor-1alpha. A pseudogene of this gene is located on the long arm of chromosome 9. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
27.9 kDa |
AA Sequence : |
MSYDRAITVFSPDGHLFQVEYAQEA VKKGSTAVGVRGRDIVVLGVEKKSV AKLQDERTVRKICALDDNVCMAFAG LTADARIVINRARVECQSHRLTVED PVTVEYITRYIASLKQRYTQSNGRR PFGISALIVGFDFDGTPRLYQTDPS GTYHAWKANAIGRGAKSVREFLEKN YTDEAIETDDLTIKLVIKALLEVVQ SGGKNIELAVMRRDQSLKILNPEEI EKYVAEIEKEKEENEKKKQKKASTR TRPLEQKLISEEDLAANDILDYKDD DDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |