Recombinant Human PSMB5 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human PSMB5 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : PSMB5-6693H
Product Overview : PSMB5 MS Standard C13 and N15-labeled recombinant protein (NP_002788) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 28.5 kDa
AA Sequence : MALASVLERP LPVNQRGFFG LGGRADLLDL GPGSLSDGLS LAAPGWGVPE EPGIEMLHGT TTLAFKFRHG VIVAADSRAT AGAYIASQTV KKVIEINPYL LGTMAGGAAD CSFWERLLAR QCRIYELRNK ERISVAAASK LLANMVYQYK GMGLSMGTMI CGWDKRGPGL YYVDSEGNRI SGATFSVGSG SVYAYGVMDR GYSYDLEVEQ AYDLARRAIY QATYRDAYSG GAVNLYHVRE DGWIRVSSDN VADLHEKYSG STPTRTRPLE QKLISEEDLA ANDILDYKDD DDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : PSMB5 proteasome 20S subunit beta 5 [ Homo sapiens (human) ]
Official Symbol : PSMB5
Synonyms : PSMB5; proteasome (prosome, macropain) subunit, beta type, 5; proteasome subunit beta type-5; MB1; X; proteasome chain 6; proteasome subunit X; proteasome subunit MB1; macropain epsilon chain; proteasome epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome subunit, beta type, 5; PSX large multifunctional protease X; multicatalytic endopeptidase complex epsilon chain; LMPX; MGC104214;
Gene ID : 5693
mRNA Refseq : NM_002797
Protein Refseq : NP_002788
MIM : 600306
UniProt ID : P28074

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